Phytochemical Methods A Guide to Modern Techniques of Plant Analysis

Author: A.J. Harborne

Publisher: Springer Science & Business Media

ISBN: 9780412572708

Category: Science

Page: 302

View: 2891

This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations. Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts. This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.
Posted in Science

Thin Layer Chromatography in Phytochemistry

Author: Monika Waksmundzka-Hajnos,Joseph Sherma,Teresa Kowalska

Publisher: CRC Press

ISBN: 9781420046786

Category: Science

Page: 896

View: 5068

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.
Posted in Science

Flavonoids and Other Polyphenols

Author: N.A

Publisher: Elsevier

ISBN: 0080496873

Category: Medical

Page: 448

View: 4713

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This volume presents an extensive collection of new methodologies to aid progress in solving unanswered questions concerning the bioavailability and metabolism of flavonoids and polyphenols, their biochemical and molecular biological effects on cell regulation, and their effects on health. Major topics in this volume include sources, characterization, analytical methods, bioavailability, antioxidant action, and biological activity.
Posted in Medical

Phytochemical Methods

A Guide to Modern Techniques of Plant Analysis

Author: J. B. Harborne

Publisher: Springer Science & Business Media

ISBN: 9400955707

Category: Juvenile Nonfiction

Page: 288

View: 4339

Posted in Juvenile Nonfiction

Flavonoids in Health and Disease, Second Edition,

Author: Catherine A. Rice-Evans,Lester Packer

Publisher: CRC Press

ISBN: 0824755243

Category: Medical

Page: 504

View: 7252

Revised and expanded throughout, this blue-ribbon reference emphasizes the latest developments in the identification, utilization, and analysis of flavonoids for the prevention of disease and maintenance of good health-examining the processes involved in the absorption, metabolism, distribution, and excretion of these compounds and the impact of biotransformation on flavonoid function.
Posted in Medical

The Systematic Identification of Flavonoids

Author: Tom Mabry,K. R. Markham,M. B. Thomas

Publisher: Springer Science & Business Media

ISBN: 364288458X

Category: Medical

Page: 354

View: 1399

About 1958, the late Professor R. E. ALSTON and Professor B. L. TURNER, both of the Department ofBotany, The University ofTexas at Austin, initiated a general systematic investigation ofthe legurne genus Baptisia. They found that flavonoid patterns, as revealed by two-dimensional paper chromatography, were valid criteria for the recognition of the Baptisia species and for the documentation of their numerous natural hybrids. Later, they showed that the flavonoid chemistry could be used for the analysis of gene flow among populations. At that time no attempt was made to even partially identify the flavonoids which were detected chromatographically. Neverthe1ess, it soon became apparent that the full value of the chemical data for systematic purposes required knowledge of the structures of the flavonoids. In 1962, one of us (T.J.M.) in collaboration with Drs. ALSTON and TURNER beg an the chemical analysis of the more than 60 flavonoids which had been chromatographi cally detected in the 16 Baptisia species. In the intervening years, a number of chemists and botanists, inc1uding Drs. K. BAETCKE, B. BREHM, M. CRANMER, D. HORNE, J. KAGAN, B. KROSCHEWSKY, J. MCCLURE, H. RÖSLER, and J. WALLACE, participated in the devel opment of techniques and procedures for the rapid identification of known flavonoids and in the structure determination of new flavonoids. In addition, the flavonoid chem istry of many plants other than Baptisia was investigated.
Posted in Medical


Chemistry, Biochemistry and Applications

Author: Oyvind M. Andersen,Kenneth R. Markham

Publisher: CRC Press

ISBN: 142003944X

Category: Science

Page: 1256

View: 4135

Advances in the flavonoid field have been nothing short of spectacular over the last 20 years. While the medical field has noticed flavonoids for their potential antioxidant, anticancer and cardioprotectant characteristics, growers and processors in plant sciences have utilized flavonoid biosynthesis and the genetic manipulation of the flavonoid pathway in plants to improve the nutritional and ornamental value of crops. Flavonoids: Chemistry, Biochemistry and Applications covers each class of flavonoid and presents the historic advances made in flavonoid research since the 1994 publication of an earlier text, Flavonoids Advances in Research Since 1986. This book details the analytical techniques scientists have used to achieve an improved understanding of flavonoid structures and functions as well as advances in the genetic manipulation of the flavonoid pathway, and the discovery of many new flavonoids. It indicates which techniques are best suited for the isolation and structure determination of flavonoids and whether the structures are novel. While explaining how to evaluate the flavonoid content in food and beverages, the book reveals the biotechnological advances that have allowed nutritionists and plant physiologists to assess the possible effects of flavonoids. As interest regarding the impact and health benefits of flavonoids continues to grow, Flavonoids: Chemistry, Biochemistry and Applications reflects the continuing commitment of flavonoid researchers to the improvement of human health and provides the most comprehensive, up-to-date source of information for all known flavonoids.
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Encyclopedia of Chromatography

Author: Jack Cazes

Publisher: CRC Press

ISBN: 9780824727871

Category: Science

Page: 800

View: 522

A guide to specific chromatographic techniques and principles. It offers basic introduction to the science and technology of the method, as well as references on the theory and methodology for analysis of specific chemicals and applications in a range of industries. It contains over 400 cross-referenced articles with more than 80 articles.
Posted in Science

Introduction to Flavonoids

Author: Bohm

Publisher: CRC Press

ISBN: 9789057023538

Category: Science

Page: 503

View: 3546

Offering a wide ranging view of this important class of plant pigments, after a brief examination of the history and literature of flavonoids, this book explores structural variation of all subclasses of flavonoids, techniques for isolation, purification, and determination of structures, chemical syntheses, biosynthesis and genetics, patterns of distribution in the plant kingdom, and uses. Finally, the functions of flavonoids in nature are investigated, as well as ways in which these compounds may have a more direct impact upon the human race.
Posted in Science

Structure information from HPLC and on-line measured absorption spectra: Flavones, Flavonols and Phenolic Acids

Author: Maria da Graça Campos,Kenneth R. Markham

Publisher: Imprensa da Universidade de Coimbra / Coimbra University Press

ISBN: 9898074051


Page: 118

View: 4915

The tools used in scientific research are constantly changing and HPLC with photodiode array detection technique has become an important and popular technology used in studies such as plant extract analysis, drug discovery and development, the monitoring of manufacturing processes and quality assurance. The HPLC/photodiode array technique is also an important base tool of pure science. The authors have provided a compilation of valuable and unique reference material, gathered using strictly controlled conditions, and have developed theoretical rules that will aid users of this technique in the identification of the structures of the titled compound types. These compound types are widely encountered in bioactive plant components. The book will have appeal not only to academic chemists but also for example to biologists, food technologists, pharmacologists, pharmacists, the health supplements industry and the colourant industry. To our knowledge this is the first book of its kind to present and interpret a compilation of actual spectra of a series of authentic compounds, measured under identical and defined conditions that practitioners can use for direct comparison with their own data.
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Handbook of Chemical and Biological Plant Analytical Methods, 3 Volume Set

Author: Shilin Chen,Andrew Marston,Hermann Stuppner

Publisher: John Wiley & Sons

ISBN: 1118874986

Category: Medical

Page: 1176

View: 3824

Plants and plant-derived compounds and drugs are becoming moreand more popular with increasing numbers of scientists researchingplant analysis. The quality control of herbal drugs is alsobecoming essential to avoid severe health problems, and in thefuture many more new drugs will be developed from plantsources. This three-volume Handbook, featuring 47 detailed review articles,is unique as it deals with chemical and biological methodologiesfor plant analysis. It presents the most important and mostaccurate methods which are available for plant analysis. This comprehensive work is divided into six sections asfollows: Sample preparation and identification – discussingplant selection and collection, followed by extraction and samplepreparation methodologies. Extraction and sample preparation methodologies Instrumentation for chemical analysis - severalinstrumentations for chemical plant analysis are presented with anemphasis on hyphenated techniques, e.g. the coupling between HPLCand mass spectroscometry, and HPLC with NMR. Strategies for selective classes of compounds –coverage of the most interesting classes of compounds such aspolysaccharides, saponins, cardiotonic glycosides, alkaloids,terpenoids, lipids, volatile compounds and polyphenols (flavonoids, xanthones, coumarins, naphthoquinones, anthraquinones,proanthocyanidins, etc.). Biological Analysis - includes phenotyping, DNA barcodingtechniques, transcriptome analysis , microarray, metabolomics andproteomics. Drugs from Plants – covers the screening of plantextracts and strategies for the quick discovery of novelbioactive natural products. Safety assessment of herbal drugs ishighly dependent on outstanding chromatographic and spectroscopicmethods which are also featured here. This Handbook introduces to scientists involved in plant studiesthe current knowledge of methodologies in various fields ofchemically- and biochemically-related topics in plantresearch. The content from this Handbook will publish online within theEncyclopedia of Analytical Chemistry via Wiley Online Library: ahref="" Benefit from the introductory offer, valid until 30 November2014! Introductory price: £425.00 / $695.00 /€550.00 List price thereafter: £495.00 / $795.00 / €640.00
Posted in Medical

Zeitschrift Für Naturforschung

A journal of chemical sciences

Author: N.A

Publisher: N.A


Category: Chemistry

Page: N.A

View: 9810

Posted in Chemistry

Isolation, Identification and Characterization of Allelochemicals/ Natural Products

Author: Diego A Sampietro,Cesar A.N. Catalan,Marta A. Vattuone

Publisher: CRC Press

ISBN: 9781578085774

Category: Science

Page: 555

View: 5570

A lot of allelopathy research has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various research methods used. To make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. This book has been written to provide information about all aspects of Allelochemicals/Natural Products. The book is divided in 3 sections Sample Collection, Handling and Storage; Isolation, Identification and Structural Elucidation and Biological Activity of Natural Products. This book will serve as ready reference in the laboratory or class room to undergraduate and graudate students and Organic Chemists for Isolation, Identification and Characterization of Allelochemicals/Natural Products. The editors have tried to provide appropriate solutions to the problems of Isolation, Identification and Characterization of Allelochemicals/Natural Products. The users of this book can select suitable methods, according to the available facilities.
Posted in Science

Flavonoids in health and disease

Author: Catherine Rice-Evans,Lester Packer

Publisher: CRC


Category: Medical

Page: 541

View: 1159

Presents the latest advances in the rapidly expanding area of research into flavonoids--discussing the molecular, biochemical, & physiological effects of flavonioids in vivo. Highlights the anticancer properties of flavonoids & investigates flavonoid influence on coronary heart disease. Furnishes evidence for the protective effects of dietary phytochemicals against chronic diseases.
Posted in Medical

Natural Products

Chemistry, Biochemistry and Pharmacology

Author: Goutam Brahmachari

Publisher: Alpha Science International Limited


Category: Science

Page: 822

View: 5830

Natural Products: Chemistry, Biochemistry and Pharmacology attempts to provide an insight into significant developments in some of the promising areas of natural products chemistry. Natural products are of great interest and promise in the present day research directed towards drug design and discovery. This book brings together, in twenty-one chapters, by leading scientists of the world, an overview of current discoveries and trends in this remarkable field. The topics, ranging from natural products chemistry and phytochemistry in their most basic form to molecular biology, pharmacology and in silico drug design, summarize years of extensive research in each area, and provide insight in the new themes of natural products research. The book serves as a valuable resource for researchers in their own fields to predict promising leads for developing pharmaceuticals to treat various ailments and disease manifestations; it also motivates young scientists to the dynamic field of bioactive natural products research.
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Proceedings of the international conference on medicinal and aromatic plants

Budapest, Hungary, 8-11 July, 2001

Author: International Society for Horticultural Science. Section on Medicinal and Aromatic Plants

Publisher: N.A


Category: Aromatic plants

Page: 331

View: 1697

Posted in Aromatic plants

Studies in natural products chemistry

Structure elucidation, part B.

Author: Atta-ur-, Rahman

Publisher: N.A

ISBN: 9780444883360

Category: Science

Page: 906

View: 9748

Posted in Science