A Guide to Modern Techniques of Plant Analysis
Author: Jeffrey B. Harborne
Publisher: Springer Science & Business Media
Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.
Author: Monika Waksmundzka-Hajnos,Joseph Sherma,Teresa Kowalska
Publisher: CRC Press
Author: Tom Mabry,K. R. Markham,M. B. Thomas
Publisher: Springer Science & Business Media
Revised and expanded throughout, this blue-ribbon reference emphasizes the latest developments in the identification, utilization, and analysis of flavonoids for the prevention of disease and maintenance of good health-examining the processes involved in the absorption, metabolism, distribution, and excretion of these compounds and the impact of biotransformation on flavonoid function.
Author: Catherine A. Rice-Evans,Lester Packer
Publisher: CRC Press
Advances in the flavonoid field have been nothing short of spectacular over the last 20 years. While the medical field has noticed flavonoids for their potential antioxidant, anticancer and cardioprotectant characteristics, growers and processors in plant sciences have utilized flavonoid biosynthesis and the genetic manipulation of the flavonoid pathway in plants to improve the nutritional and ornamental value of crops. Flavonoids: Chemistry, Biochemistry and Applications covers each class of flavonoid and presents the historic advances made in flavonoid research since the 1994 publication of an earlier text, Flavonoids Advances in Research Since 1986. This book details the analytical techniques scientists have used to achieve an improved understanding of flavonoid structures and functions as well as advances in the genetic manipulation of the flavonoid pathway, and the discovery of many new flavonoids. It indicates which techniques are best suited for the isolation and structure determination of flavonoids and whether the structures are novel. While explaining how to evaluate the flavonoid content in food and beverages, the book reveals the biotechnological advances that have allowed nutritionists and plant physiologists to assess the possible effects of flavonoids. As interest regarding the impact and health benefits of flavonoids continues to grow, Flavonoids: Chemistry, Biochemistry and Applications reflects the continuing commitment of flavonoid researchers to the improvement of human health and provides the most comprehensive, up-to-date source of information for all known flavonoids.
Chemistry, Biochemistry and Applications
Author: Oyvind M. Andersen,Kenneth R. Markham
Publisher: CRC Press
The tools used in scientific research are constantly changing and HPLC with photodiode array detection technique has become an important and popular technology used in studies such as plant extract analysis, drug discovery and development, the monitoring of manufacturing processes and quality assurance. The HPLC/photodiode array technique is also an important base tool of pure science. The authors have provided a compilation of valuable and unique reference material, gathered using strictly controlled conditions, and have developed theoretical rules that will aid users of this technique in the identification of the structures of the titled compound types. These compound types are widely encountered in bioactive plant components. The book will have appeal not only to academic chemists but also for example to biologists, food technologists, pharmacologists, pharmacists, the health supplements industry and the colourant industry. To our knowledge this is the first book of its kind to present and interpret a compilation of actual spectra of a series of authentic compounds, measured under identical and defined conditions that practitioners can use for direct comparison with their own data.
Author: Maria da Graça Campos,Kenneth R. Markham
Publisher: Imprensa da Universidade de Coimbra / Coimbra University Press
Plants and plant-derived compounds and drugs are becoming more and more popular with increasing numbers of scientists researching plant analysis. The quality control of herbal drugs is also becoming essential to avoid severe health problems, and in the future many more new drugs will be developed from plant sources. This three-volume Handbook, featuring 47 detailed review articles, is unique as it deals with chemical and biological methodologies for plant analysis. It presents the most important and most accurate methods which are available for plant analysis. This comprehensive work is divided into six sections as follows: Sample preparation and identification – discussing plant selection and collection, followed by extraction and sample preparation methodologies. Extraction and sample preparation methodologies Instrumentation for chemical analysis - several instrumentations for chemical plant analysis are presented with an emphasis on hyphenated techniques, e.g. the coupling between HPLC and mass spectroscometry, and HPLC with NMR. Strategies for selective classes of compounds – coverage of the most interesting classes of compounds such as polysaccharides, saponins, cardiotonic glycosides, alkaloids, terpenoids, lipids, volatile compounds and polyphenols ( flavonoids, xanthones, coumarins, naphthoquinones, anthraquinones, proanthocyanidins, etc.). Biological Analysis - includes phenotyping, DNA barcoding techniques, transcriptome analysis , microarray, metabolomics and proteomics. Drugs from Plants – covers the screening of plant extracts and strategies for the quick discovery of novel bioactive natural products. Safety assessment of herbal drugs is highly dependent on outstanding chromatographic and spectroscopic methods which are also featured here. This Handbook introduces to scientists involved in plant studies the current knowledge of methodologies in various fields of chemically- and biochemically-related topics in plant research. The content from this Handbook will publish online within the Encyclopedia of Analytical Chemistry via Wiley Online Library: http://www.wileyonlinelibrary.com/ref/eac Benefit from the introductory offer, valid until 30 November 2014! Introductory price: £425.00 / $695.00 / €550.00 List price thereafter: £495.00 / $795.00 / €640.00
Author: Shilin Chen,Andrew Marston,Hermann Stuppner
Publisher: John Wiley & Sons
A journal of chemical sciences
A lot of allelopathy research has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various research methods used. To make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. This book has been written to provide information about all aspects of Allelochemicals/Natural Products. The book is divided in 3 sections Sample Collection, Handling and Storage; Isolation, Identification and Structural Elucidation and Biological Activity of Natural Products. This book will serve as ready reference in the laboratory or class room to undergraduate and graudate students and Organic Chemists for Isolation, Identification and Characterization of Allelochemicals/Natural Products. The editors have tried to provide appropriate solutions to the problems of Isolation, Identification and Characterization of Allelochemicals/Natural Products. The users of this book can select suitable methods, according to the available facilities.
Author: Diego A Sampietro,Cesar A.N. Catalan,Marta A. Vattuone
Publisher: CRC Press
Presents the latest advances in the rapidly expanding area of research into flavonoids--discussing the molecular, biochemical, & physiological effects of flavonioids in vivo. Highlights the anticancer properties of flavonoids & investigates flavonoid influence on coronary heart disease. Furnishes evidence for the protective effects of dietary phytochemicals against chronic diseases.
Author: Catherine Rice-Evans,Lester Packer
Natural Products: Chemistry, Biochemistry and Pharmacology attempts to provide an insight into significant developments in some of the promising areas of natural products chemistry. Natural products are of great interest and promise in the present day research directed towards drug design and discovery. This book brings together, in twenty-one chapters, by leading scientists of the world, an overview of current discoveries and trends in this remarkable field. The topics, ranging from natural products chemistry and phytochemistry in their most basic form to molecular biology, pharmacology and in silico drug design, summarize years of extensive research in each area, and provide insight in the new themes of natural products research. The book serves as a valuable resource for researchers in their own fields to predict promising leads for developing pharmaceuticals to treat various ailments and disease manifestations; it also motivates young scientists to the dynamic field of bioactive natural products research.
Chemistry, Biochemistry and Pharmacology
Author: Goutam Brahmachari
Publisher: Alpha Science International Limited
Structure elucidation, part B.
Author: Atta-ur-, Rahman
The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field. The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products. Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.
Author: Monika Waksmundzka-Hajnos,Joseph Sherma
Publisher: CRC Press
Phytochemicals are the individual chemicals from which the plants are made and plants are the key sources of raw material for both pharmaceutical and aromatic industries. the improved methods for higher yield of active compounds will be the major incentive in these industries. To help those who are involved in the isolation of compounds from plants, some of the essential phytochemical techniques are included in this book. The theoretical principles of various instruments, handling of samples and interpretation of spectra are given in detail. Adequate chemical formulas are included to support and explain various structures of compounds and techniques. The book will prove useful to students, researchers, professionals in the field of Plant Physiology and Pathology, Pharmaceutical and Chemical Engineering, Biotechnology, Medicinal and Aromatic Plants and Horticulture.
Author: N. Raaman
Publisher: New India Publishing
Author: Gerardo Rodríguez