Pumps, Channels and Transporters

Methods of Functional Analysis

Author: Ronald J. Clarke,Mohammed A. A. Khalid

Publisher: John Wiley & Sons

ISBN: 1118858808

Category: Medical

Page: 488

View: 2034

"Ion-transporting membrane proteins permanently embedded in the membranes of cells or cell organelles can be grouped into three broad categories: channels, pumps, and transporters"--Chapter 1, introduction.
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The Red Cell Membrane

A Model for Solute Transport

Author: B. U. Raess,G. Tunnicliff

Publisher: Springer Science & Business Media

ISBN: 1461245001

Category: Science

Page: 476

View: 534

"After being frequently urged to write upon this subject, and as often declining to do it, from apprehension of my own inability, I am at length compelled to take up the pen, however unqualified I may still feel myself for the task. " William Withering, M. D. ' I have yet to find a description or a quote that better summanzes my initial ambivalence towards embarking on such an endeavor as partici pating in putting together this monograph. The impetus for The Red-Cell has been a simple, genuine Membrane: A Model for Solute Transport desire to bring together an authoritative account of the' 'state of the art and knowledge" in the red-ceIl-membrane transport field. In particular, it seems important to emphasize the pivotal role the red cell has played for several decades in the discovery and the elucidation of mechanisms of plasma-membrane transport processes. It is only with such knowledge that we can hope to push ahead and make progress in this exciting, multifaceted area. Eventually, one hopes to not only further our knowledge of red cells, but apply the newly gained insights to any other of the plasma membrane. cell with the common denominator In this compendium of reviews, the reader will find that the term model will take on a variety of gists and meanings. In some chapters, the red cell has been chosen as a model membrane solely on the basis of its preeminent design and simplicity.
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Red Cell Membrane Transport in Health and Disease

Author: Ingolf Bernhardt,J. Clive Ellory

Publisher: Springer Science & Business Media

ISBN: 3662051818

Category: Science

Page: 748

View: 4749

51 worldwide leading experts in the field of erythrocyte research contributed to this first book on transport processes in red blood cells. It explains the latest findings on the basis of well-established principles, in an accessibly structured and carefully organized compilation.
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Calcium signalling

Approaches and Findings in the Heart and Blood

Author: Lars Kaestner

Publisher: Springer Science & Business Media

ISBN: 3642346170

Category: Medical

Page: 239

View: 6776

The book combines general concepts and methods to investigate calcium signalling in cells ranging from molecular biology approaches to manipulation of calcium in living cells. The focus within these methods in on the broad range of fluorescence imaging technology, in particular on optical sectioning techniques and fast image acquisition. In addition to these general guidelines there are application examples in a context beyond calcium signalling in two major fields: investigations of isolated cardiac myocytes and red blood cell related research. While the cellular cardiology section provides snapshots of certain calcium signalling aspects, the red blood cell part presents an overview from the functional identification of calcium-channels to a concept of physiological and pathophysiological relevance.
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Erythroid Cells

Author: J. Robin Harris

Publisher: Springer Science & Business Media

ISBN: 9780306434624

Category: Medical

Page: 537

View: 3761

This series of books, devoted to aspects of blood cell biochemistry, development, immu nology, and ultrastructure, has evolved and separated from the long-established Plenum series Subcellular Biochemistry. It is the intention of these volumes to draw together related areas of investigation and to provide, in the fullness of time, complete coverage of this rapidly advancing important biomedical discipline. Both fundamental and medically applied topics, dealing with normal and pathological cells, will be included. This, the first volume of the series, contains a diverse collection of chapters, all of which relate to erythroid cells. The range of material included is extremely broad and the authors have used contrasting technical approaches, both within their personal experimen tal studies and within their manuscripts. This has led to the production of a very interest ing compilation, which does, nevertheless, possess a strong overall thematic unity. As with all edited volumes, some topics of importance and interest are not included. This may be because of oversight on my part, as editor, or because the authors originally selected failed to submit their manuscript by the agreed-upon submission date. For these omissions I take full responsibility and trust that at least some of the topics omitted, for instance membrane cation transport systems, will be covered within a future volume of the series. This book commences with two chapters of a developmental nature.
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Pulmonary Arterial Hypertension, An Issue of Clinics in Chest Medicine,

Author: Terence K. Trow

Publisher: Elsevier Health Sciences

ISBN: 0323277934

Category: Medical

Page: 886

View: 1833

Guest editor Terence K. Trow has assembled an expert team of authors on the topic of Pulmonary Arterial Hypertension. Articles include: Epidemiology of Pulmonary Arterial Hypertension, Pathology of Pulmonary Hypertension, Genetics of Pulmonary Arterial Hypertension, Diagnosis of Pulmonary Arterial Hypertension, Pulmonary Hypertension Owing to Left Heart Disease, Pulmonary Hypertension due to Lung Disease and/or Hypoxia, Pulmonary Arterial Hypertension Associated with Congenital Heart Disease, World Health Organization Group 5 Pulmonary Hypertension, and more!
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Cell Separation

Methods and Selected Applications

Author: Thomas G. Pretlow,Theresa P. Pretlow

Publisher: Academic Press

ISBN: 1483219380

Category: Science

Page: 342

View: 2933

Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.
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Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book

Author: Richard A. McPherson,Matthew R. Pincus

Publisher: Elsevier Health Sciences

ISBN: 1455726842

Category: Medical

Page: 1568

View: 9506

Recognized as the definitive book in laboratory medicine since 1908, Henry’s Clinical Diagnosis and Management by Laboratory Methods, edited by Richard A. McPherson, MD and Matthew R. Pincus, MD, PhD, is a comprehensive, multidisciplinary pathology reference that gives you state-of-the-art guidance on lab test selection and interpretation of results. Revisions throughout keep you current on the latest topics in the field, such as biochemical markers of bone metabolism, clinical enzymology, pharmacogenomics, and more! A user-friendly full-color layout puts all the latest, most essential knowledge at your fingertips. Update your understanding of the scientific foundation and clinical application of today's complete range of laboratory tests. Get optimal test results with guidance on error detection, correction, and prevention as well as cost-effective test selection. Reference the information you need quickly and easily thanks to a full-color layout, many new color illustrations and visual aids, and an organization by organ system. Master all the latest approaches in clinical laboratory medicine with new and updated coverage of: the chemical basis for analyte assays and common interferences; lipids and dyslipoproteinemia; markers in the blood for cardiac injury evaluation and related stroke disorders; coagulation testing for antiplatelet drugs such as aspirin and clopidogrel; biochemical markers of bone metabolism; clinical enzymology; hematology and transfusion medicine; medical microbiology; body fluid analysis; and many other rapidly evolving frontiers in the field. Effectively monitor the pace of drug clearing in patients undergoing pharmacogenomic treatments with a new chapter on this groundbreaking new area. Apply the latest best practices in clinical laboratory management with special chapters on organization, work flow, quality control, interpretation of results, informatics, financial management, and establishing a molecular diagnostics laboratory. Confidently prepare for the upcoming recertification exams for clinical pathologists set to begin in 2016.
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Methodologies for Metabolomics

Experimental Strategies and Techniques

Author: Norbert W. Lutz,Jonathan V. Sweedler,Ron A. Wevers

Publisher: Cambridge University Press

ISBN: 0521765900

Category: Medical

Page: 627

View: 8273

Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.
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Introduction to the Blood-Brain Barrier

Methodology, Biology and Pathology

Author: William M. Pardridge

Publisher: Cambridge University Press

ISBN: 9780521581240

Category: Medical

Page: 486

View: 8901

The blood-brain barrier serves to protect the brain from toxic substances whilst simultaneously allowing access to essential nutrients and chemical signals. At the interface between brain and body, knowledge of the blood-brain barrier forms an essential component in the complete understanding of a large proportion of medical disciplines. Nevertheless, it seems that ignorance of both the biology of this important membrane and the methodology suitable for its investigation still remains an impediment to progress in many fields, including, for example, the development of new and efficacious neuropharmaceuticals, cerebrovascular disease, Alzheimer's disease, cerebral AIDS and brain tumours. This introduction for both researchers and clinicians across the medical sciences is intended to aid both those beginning work directly in this area and those wishing simply to be better informed when interpreting information where the blood-brain barrier may be involved. Advances in both methodology and biology are detailed in 50 chapters from international authorities.
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Biomedical EPR - Part B: Methodology, Instrumentation, and Dynamics

Author: Sandra S. Eaton,Gareth R. Eaton,Lawrence J. Berliner

Publisher: Springer Science & Business Media

ISBN: 9780306485329

Category: Medical

Page: 470

View: 2656

Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features:Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation About the Editors: Prof. Sandra S. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. Her research interests include distance measurements in proteins, EPR of metal ions in biological systems, electron spin relaxation times, and EPR instrumentation. The Eatons co-organize an annual EPR Symposium in Denver. Prof. Gareth R. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. His research interests include EPR instrumentation, distance measurements in proteins, EPR of metal ions in biological systems, and electron spin relaxation times. Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.
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Accurate Results in the Clinical Laboratory

A Guide to Error Detection and Correction

Author: Amitava Dasgupta,Jorge L. Sepulveda

Publisher: Newnes

ISBN: 0124158587

Category: Science

Page: 382

View: 7233

This practical, easy-to-use guide, named to Doody’s Core Titles 2013, addresses interference issues in all laboratory tests, including patient epigenetics, process of specimen collection, enzymes, biomarkers. Clinicians and laboratory scientists can therefore rely on one reference which speaks to both their needs of accurate specimen analysis and optimal patient care. Erroneous hospital and pathology laboratory results can be confusing and problematic, especially in acute care situations. While some factors creating interference, can be identified in the laboratory, detecting many others is often dependent on clinical details unavailable to the laboratory scientists or pathologists. Therefore, clinicians must become proficient in identifying such erroneous reports, and working with pathologists and laboratory scientists so that they can understand the source of such interferences, correct the results, and then decide what course of action must be followed for proper patient management. Named to Doody’s Core Titles 2013, a collection development tool for health sciences libraries of all sizes, by Doody Enterprises Practical information for both clinicians and laboratory scientists, presented in the form of tables and charts for easy reference Focus on range and sources of interferences rather than details of toxicologic mechanisms which are well covered in toxicology textbooks Covers interferences across endocrine, oncology, hematology, immunohistochemistry, immunology, serology, microbiology, and molecular testing
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Nutrition Research Methodologies

Author: Julie A. Lovegrove,Leanne Hodson,Sangita Sharma,Susan A. Lanham-New

Publisher: John Wiley & Sons

ISBN: 1118554671

Category: Medical

Page: 360

View: 8532

Nutrition Research Methodologies Edited by Julie A Lovegrove, Leanne Hodson, Sangita Sharma and Susan A Lanham-New A new book in the acclaimed Nutrition Society Textbook Series, "Nutrition Research Methodologies" addresses the rapidly advancing field of nutrition research. It covers the diverse methodologies required for robust nutritional research to ensure thorough understanding of key concepts, both for students at undergraduate and postgraduate levels and for scientists working in nutrition research. Combining theory with practical application, "Nutrition Research Methodologies" addresses both traditional research methods and new technologies, and focuses on a range of complex topics, including energy compensation, nutrient-gene interactions and metabolic adaptation. It also considers statistical issues as well as application of data to policy development. Provides the reader with the required scientific basics of nutrition research in the context of a systems and health approach Written specifically to meet the needs of individuals involved in nutrition research Combines the viewpoints of world-leading nutrition experts from academia and research with practical applications Accompanied by a companion website with a range of self-assessment material About the Editors: Professor Julie A Lovegrove, Hugh Sinclair Unit of Human Nutrition and Institute for Cardiovascular Research, Department of Food and Nutritional Sciences, University of Reading, UK Associate Professor Leanne Hodson, Oxford Centre for Diabetes, Endocrinology & Metabolism, Radcliffe Department of Medicine, University of Oxford, UK Professor Sangita Sharma, Centennial Professor, Endowed Chair in Aboriginal Health, Professor of Aboriginal & Global Health Research, Aboriginal and Global Health Research Group, Department of Medicine, Faculty of Medicine & Dentistry, University of Alberta, Canada Professor Susan A Lanham-New, Department of Nutritional Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, UK With website: A supporting companion website featuring illustrations and self-assessment questions is available at www.wiley.com/go/lovegrove/nutritionresearch Other books in the Nutrition Society Textbook Series: "Clinical Nutrition, Second Edition" ISBN: 978-1-4051-6810-6 "Sport and Exercise Nutrition " ISBN: 978-1-4443-3468-5 "Nutrition and Metabolism, Second Edition " ISBN: 978-1-4051-6808-3 "Introduction to Human Nutrition, Second Edition " ISBN: 978-1-4051-6807-6 "Public Health Nutrition" ISBN: 978-0-632-05627-9 For further information, companion material for use with these textbooks, and full details of how to purchase them, visit: www.wiley.com/go/nutritionsociety
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Small Animal Clinical Diagnosis by Laboratory Methods - E-Book

Author: Michael D. Willard,Harold Tvedten

Publisher: Elsevier Health Sciences

ISBN: 1437706584

Category: Medical

Page: 432

View: 2188

A quick guide to appropriately selecting and interpreting laboratory tests, Small Animal Clinical Diagnosis by Laboratory Methods, 5th Edition helps you utilize your in-house lab or your specialty reference lab to efficiently make accurate diagnoses without running a plethora of unnecessary and low-yield tests. It provides answers to commonly asked questions relating to laboratory tests, and solutions to frequently encountered problems in small animal diagnosis. For easy reference, information is provided by clinical presentation and abnormalities, and includes hundreds of tables, boxes, key points, and algorithms. This edition, now in full color, is updated with the latest advances in laboratory testing methods and diagnostic problem solving. Written by noted educators Dr. Michael Willard and Dr. Harold Tvedten, this book may be used as an on-the-spot guide to specific problems or conditions as well as a reference for more detailed research on difficult cases. Concise discussions address laboratory approaches to various disorders, possible conclusions from various test results, artifacts and errors in diagnoses, and interpretations leading to various diagnoses. Hundreds of tables, boxes, algorithms, and key points offer at-a-glance information including cautions, common pitfalls, and helpful "pearls," and lead to proper differential and clinical diagnostic decision making. Note boxes identify key considerations in correlating clinical signs with test data for accurate diagnoses, highlight safety precautions, and offer helpful tips for sample preparation and interpretation. Chapters on laboratory diagnostic toxicology and therapeutic drug monitoring help in handling potentially fatal poisonings and other special situations. Expert editors and contributors provide clinical knowledge and successful diagnostic problem-solving solutions. A practical appendix lists referral laboratories that may be contacted for certain diseases, and reference values with the normal or expected range for coagulation, hematology, and more. Updated coverage integrates the newest advances in testing methods and diagnostic problem solving. Full-color photos and schematic drawings are placed adjacent to related text, and accurately depict diagnostic features on microscopic slide preparations as well as test procedures and techniques.
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Laboratory Methods in Cell Biology

Biochemistry and Cell Culture

Author: N.A

Publisher: Academic Press

ISBN: 0124055494

Category: Science

Page: 544

View: 5830

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields
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Linne & Ringsrud's Clinical Laboratory Science - E-Book

The Basics and Routine Techniques

Author: Mary Louise Turgeon

Publisher: Elsevier Health Sciences

ISBN: 0323292801

Category: Medical

Page: 680

View: 6072

Updated and easy-to-use, Linne & Ringsrud's Clinical Laboratory Science: The Basics and Routine Techniques, 6th Edition delivers a fundamental overview of the laboratory skills and techniques essential for success in your classes and your career. Author Mary Louise Turgeon's simple, straightforward writing clarifies complex concepts, and a discipline-by-discipline approach helps you build the knowledge to confidently perform clinical laboratory tests and ensure accurate, effective results. Expert insight from respected educator and author Mary Louise Turgeon reflects the full spectrum of clinical laboratory science. Engaging full-color design and illustrations familiarize you with what you'll see under the microscope. Streamlined approach makes must-know concepts and practices more accessible. Broad scope provides an ideal introduction to clinical laboratory science at various levels, including MLS/MLT and Medical Assisting. Hands-on procedures guide you through the exact steps you'll perform in the lab. Learning objectives help you identify key chapter content and study more effectively. Case studies challenge you to apply concepts to realistic scenarios. Review questions at the end of each chapter help you assess your understanding and identify areas requiring additional study. A companion Evolve website provides convenient online access to procedures, glossary, audio glossary and links to additional information. Updated instrumentation coverage familiarizes you with the latest technological advancements in clinical laboratory science. Perforated pages make it easy for you to take procedure instructions with you into the lab. Enhanced organization helps you study more efficiently and quickly locate the information you need. Convenient glossary provides fast, easy access to definitions of key terms.
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Cardiovascular Fluid Mechanics

Author: Gianni Pedrizzetti,Karl Perktold

Publisher: Springer Science & Business Media

ISBN: 9783211005385

Category: Technology & Engineering

Page: 271

View: 874

The book presents the state of the art in the interdisciplinary field of fluid mechanics applied to cardiovascular modelling. It is neither a monograph nor a collection of research papers, rather an extended review in the field. It is arranged in 4 scientific chapters each presenting thoroughly the approach of a leading research team; two additional chapters prepared by biomedical scientists present the topic by the applied perspective. A unique feature is a substantial (approx. one fourth of the book) medical introductory part, written by clinical researchers for scientific readers, that would require a large effort to be collected otherwise.
Posted in Technology & Engineering

Membrane Proteins

A Laboratory Manual

Author: A. Azzi,U. Brodbeck,P. Zahler

Publisher: Springer Science & Business Media

ISBN: 3642680771

Category: Science

Page: 258

View: 8824

The growing interest in the field of biological membranes in recent years is documented by the very large number of articles, reviews, journals and books which are appearing in this field. Why then now a manual on "Membrane Proteins"? The answer is multifold. The protocols which were distributes by the teachers and lecturers at the FEBS-SKMB Course organized in Bern appeared to be very useful not only during the Course to correctly perform the experi ments, but also for the future benefit of other students and other curses. To us they appeared very modern and of simple execution, ideal for a Uni versity Advanced Course, a Summer School, or similar scientific initiatives. The possibility was also foreseen that such a manual could be used by pro fessional scientists, although not initiated into the problems, assumptions and intricacies of biochemical methodology. There are also many research teams who study proteins, for example of human fluids, and who will certainly be interested in the application of new but simply described methods. At the same time we present the student with some more complicated physical techniques which are, however, simply described and easy to execute.
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