Phytochemical Methods

A Guide to Modern Techniques of Plant Analysis

Author: Jeffrey B. Harborne

Publisher: Springer Science & Business Media

ISBN: 9400959214

Category: Science

Page: 278

View: 8107

While there are many books available on methods of organic and biochemical analysis, the majority are either primarily concerned with the application of a particular technique (e.g. paper chromatography) or have been written for an audience of chemists or for biochemists work ing mainly with animaltissues. Thus, no simple guide to modern metho ds of plant analysis exists and the purpose of the present volume is to fill this gap. It is primarily intended for students in the plant sciences, who have a botanical or a general biological background. It should also be of value to students in biochemistry, pharmacognosy, food science and 'natural products' organic chemistry. Most books on chromatography, while admirably covering the needs of research workers, tend to overwhelm the student with long lists of solvent systems and spray reagents that can be applied to each class of organic constituent. The intention here is to simplify the situation by listing only a few specially recommended techniques that have wide currency in phytochemical laboratories. Sufficient details are provided to allow the student to use the techniques for themselves and most sections contain some introductory practical experiments which can be used in classwork.
Posted in Science

Phytochemical Techniques

Author: N. Raaman

Publisher: New India Publishing

ISBN: 9788189422301

Category: Medical

Page: 320

View: 4616

Phytochemicals are the individual chemicals from which the plants are made and plants are the key sources of raw material for both pharmaceutical and aromatic industries. the improved methods for higher yield of active compounds will be the major incentive in these industries. To help those who are involved in the isolation of compounds from plants, some of the essential phytochemical techniques are included in this book. The theoretical principles of various instruments, handling of samples and interpretation of spectra are given in detail. Adequate chemical formulas are included to support and explain various structures of compounds and techniques. The book will prove useful to students, researchers, professionals in the field of Plant Physiology and Pathology, Pharmaceutical and Chemical Engineering, Biotechnology, Medicinal and Aromatic Plants and Horticulture.
Posted in Medical

Modern Phytochemical Methods

Author: Nikolaus H. Fischer,Murray B. Isman,Helen A. Stafford

Publisher: Springer Science & Business Media

ISBN: 1468490605

Category: Science

Page: 418

View: 2194

This volume contains reviews which are based on a symposium, given th at the 30 meeting of The Phytochemical Society of North America, held at Laval University in Quebec City, Canada on August 11-15, 1990. During the past two decades, there have been major new developments in methods which can be applied toward the isolation, separation and structure determination of complex natural products. Therefore, the topic of this symposium, "Modem Phytochemical Methods", is a very timely one. The organizers of the symposium recognized that it would not be possible to cover in detail all new advances in phytochemical methodology. It was therefore decided to emphasize general reviews on recent developments of major separation techniques such as high performance liquid chromatography as well as supercritical fluid chromato graphy. In addition, advances in commonly used structure determination methods, mainly NMR and MS, are reviewed. Other topics include methodo logies of micro-sampling for isolation and analysis of trichome constituents as well as recent breakthroughs on biosynthetic studies of monoterpenes using "enriched" basal cells of trichomes. The volume concludes with a review of quantitative structure-activity relationship (QSAR) studies of biologically active natural products. In Chapter I, K. Hostettmann and his colleagues give a general review of recent developments in the separation of natural products with major emphasis on preparative separations of biologically active plant constituents. The authors present a comparison of droplet countercurrent chromatography (OCCC) with the highly rapid and more versatile centrifugal partition chromatography (CPC).
Posted in Science

Natural Products Isolation

Author: Satyajit D. Sarker,Zahid Latif,Alexander I. Gray

Publisher: Springer Science & Business Media

ISBN: 1592599559

Category: Science

Page: 515

View: 3430

Natural Products Isolation: Second Edition presents a practical overview of just how natural products can be extracted, prepared, and isolated from the source material. Maintaining the main theme and philosophy of the first edition, this second edition incorporates all the new significant developments in this field of research. The chapters are divided into four distinct sections: introduction, extraction, chromatography, and special topics. This second edition provides substantial backround information for natural product researchers and will prove a useful reference guide to all of the available techniques.
Posted in Science

Experimental Phytochemical Techniques

Author: N. Raaman

Publisher: New India Publishing

ISBN: 9789380235943

Category: Medical

Page: 452

View: 9509

Papers presented at the National Conference on "Recent Trends on Medicinal Plants".
Posted in Medical

Methods in Plant Biochemistry

Author: P. M. Dey

Publisher: Academic Press

ISBN: 0080984185

Category: Science

Page: 608

View: 4265

Methods in Plant Biochemistry, Volume 1: Plant Phenolics reviews current knowledge about techniques used in the analysis of the biochemistry of plant polyphenols and their importance in the agricultural and food industries. It looks at the application of these techniques in the fractionation of cellular constituents, isolation of enzymes, electrophoretic separation of nucleic acids and proteins, and chromatographic identification of the intermediates and products of cellular metabolism. Organized into 15 chapters, this book opens with an overview of the general procedures and measurement of total phenolics, from detecting phenolic substances in crude plant extracts to determining which classes they belong to and the quantitative estimation of total phenol. The reader is introduced to the chemistry, structural variation, function, and distribution of each class of plant phenolics and, in a few cases where this is practicable, detailed listings of known derivatives are given. Most chapters focus on chromatographic separations and high performance liquid chromatography (HPLC), along with thin layer and paper Rf values with HPLC retention times and NMR spectroscopy. The book also outlines the procedures for the extraction, isolation, separation, and characterization of different classes of phenolic compounds, ranging from phenols and phenolic acids to phenylpropanoids, lignins, stilbenes and phenanthrenes, flavones and flavonols, chalcones and aurones, flavanoids, anthocyanins, biflavanoids, tannins, isoflavanoids, quinones, xanthones, and lichen substances. The book is a valuable resource for students, biochemists, and researchers in the plant sciences.
Posted in Science

Fundamentals of Pharmacognosy and Phytotherapy E-Book

Author: Michael Heinrich,Joanne Barnes,Simon Gibbons,Elizabeth M. Williamson

Publisher: Elsevier Health Sciences

ISBN: 0702052310

Category: Medical

Page: 336

View: 2804

‘A new classic’ in a new edition! Fully revised and updated throughout New sections on antimicrobials From journal reviews of the previous edition: ‘Drawing on their wealth of experience and knowledge in this field, the authors, who are without doubt among the finest minds in pharmacognosy today, provide useful and fascinating insights into the history, botany, chemistry, phytotherapy and importance of medicinal plants in some of today's health care systems. This is a landmark textbook, which carefully brings together relevant data from numerous sources and provides in an authoritative and exhaustive manner, cutting edge information that is relevant to pharmacists, pharmacognocists, complementary practitioners, doctors and nurses alike.’ The Pharmaceutical Journal ‘This is the first book that I have encountered which combines the compounds and plants found in standard pharmacognosy textbooks, i.e. those used in orthodox Western medicine, with the 'new phytopharmaceuticals' which have become established in Western culture over the last 20 years. The medical establishment in this environment is finally catching up with the practices of the general population and so this book is an excellent choice for those who wish to investigate which of the many plants available have some scientific credence. I shall be adding this book to the Essential Reading list for all of the undergraduate students on our pharmacy degree course and would encourage all those involved in teaching pharmacy students to do the same." P.J. Houghton, Department of Pharmacy, King's College London, Journal of Ethnopharmacology ‘Educated pharmacists no doubt equate Pharmacognosy with hours spent hunched over a microscope identifying vegetable drugs. Many probably consider it as a subject with little importance in a modern pharmacy curriculum. How wrong they are! ... This book is designed to give an overview at an easy-to-understand level of a broad subject area... For students of science and of the healthcare professions it is a useful text and the authors are to be commended for their work.’ Irish Pharmacy Journal From customer reviews: ‘A new classic. This is an excellent publication both for science students and the non scientific who have an interest in phytotherapy. The layout is logical and clearly set out. I love the chemical structural diagrams, and the explanations of even complex sequences are easy to understand with very little jargon. It is encouraging to see pharmacognosy being given a prominent place in a modern textbook, and interesting to see both hand drawings and chemical structures on the same page!’ ‘I can recommend this to anyone who is interested in the science behind herbal products and medicines; especially if you are interested in plants. It's quite simple to follow and very concise! Good for pharmacy students.’ ‘This is an ultimate textbook in this subject and a boon for students of M Pharmacy (Pharmacognosy) as well as undergraduates students of Pharmacy. Besides them, it is really suitable for every course comprising a study of plants and their medicinal use.’ ‘Excellent reference book. As an editor, I instantly found the answers to various questions I had regarding botanical descriptions. And it even answered questions that I hadn't gotten around to asking. Highly recommended!’
Posted in Medical

Chromatographic Methods

Author: A. Braithwaite

Publisher: Springer Science & Business Media

ISBN: 9400940939

Category: Science

Page: 414

View: 7884

In recent years the techniques of chromatography have progressed rapidly. However, the aims and objectives of the First Edition, as quoted below, are just as relevant today as they undoubtedly were in 1963. 'The various methods of separating mixtures which are grouped under the general name chromatography are now well known and widely used. Since the inception of chromatography as a column technique in 1903, the principal landmarks in its progress have been its virtual rediscovery in the 1930s, the invention of synthetic resins in 1935, the introduction of paper chromatography in the early 1940s and finally, the development of gas solid and gas liquid chromatography in the late 1940s and early 1950s. Subsequent expansion in the use of chromatographic methods has been rapid and continuous, with the result that in the last 15 years a substantial volume of literature on the subject has appeared, dealing not only with particular separations but also in much specific detail with improvements in technique.
Posted in Science

Ökologische Biochemie

Eine Einführung

Author: Jeffrey B. Harborne

Publisher: Springer Spektrum

ISBN: 9783642398506

Category: Science

Page: 383

View: 8504

Didaktisch geschickt und anschaulich werden die biochemischen Beziehungen zwischen Pflanzen, Tieren und ihrer Umwelt beschrieben. Das Buch ist auch für Leser mit geringen Biochemiekenntnissen leicht verständlich. Aus dem Inhalt: Pflanzen und biochemische Adaption an Umwelt Biochemie der Pflanzenbestäubung Pflanzentoxine und ihre Wirkung auf Tiere Hormonelle Beziehungen zwischen Pflanzen und Tieren Nahrungspräferenzen Abwehrmechanismen von Pflanzen und Reaktionen der Tiere (Coevolution) Pheromone: Biochemische Kommunikation zwischen Tieren Biochemische Wechselwirkungen zwischen Pflanzen.
Posted in Science

Phytochemical Analysis

(A Brief Guide of Methods Used in Phytochemistry Research)

Author: Dr. Vinesh Kumar

Publisher: Booktango

ISBN: 1468972111

Category: Education

Page: N.A

View: 3817

The aim of this book is to provide the brief introduction of the techniques used for phytochemical studies. This book includes the methods used for plant material collection, their storage, extraction, isolation, and identification of organic constituents present in plant materials under study.
Posted in Education

Molecular Biology

Author: N.A

Publisher: Academic Press

ISBN: 9780080551760

Category: Science

Page: 126

View: 7803

Methods in Plant Biochemistry is an authoritative reference on current techniques in the various fields of plant biochemical research. Under the expert guidance of guest editors, each volume provides comprehensive, practical information on the assay and analytical techniques appropriate for a particular family of plant compounds. The series emphasizes techniques and laboratory procedures. No plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference.
Posted in Science

Enzymes of Primary Metabolism

Author: N.A

Publisher: Academic Press

ISBN: 0080984215

Category: Science

Page: 432

View: 8334

The series Methods in Plant Biochemistry provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. The historical background and current, most useful methods of analysis are described. Detailed discussions of the protocols and suitability of each technique are included. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature.**Conceived as a practical comparison to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source.
Posted in Science

Carbohydrates

Author: N.A

Publisher: Academic Press

ISBN: 0080984207

Category: Science

Page: 672

View: 3631

The series, Methods in Plant Biochemistry, provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. Each will describe the historical background and current, most useful methods of analysis. The volumes include detailed discussions of the protocols and suitability of each technique. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature. Conceived as a practical companion to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source.
Posted in Science

Spektroskopische Methoden in der organischen Chemie

Author: Manfred Hesse,Herbert Meier,Bernd Zeeh

Publisher: Georg Thieme Verlag

ISBN: 9783135761077

Category: Organic compounds

Page: 456

View: 8952

Posted in Organic compounds

Pflanzenbiochemie

Author: Hans Walter Heldt,Birgit Piechulla

Publisher: Springer-Verlag

ISBN: 3662443988

Category: Science

Page: 597

View: 4301

Die „Pflanzenbiochemie" hat sich im deutschsprachigen Raum, aber auch in zahlreichen Übersetzungen als Standardlehrbuch etabliert. Birgit Piechulla, Dozentin an der Universität Rostock, zeichnet als Co-Autorin bei dieser 5. Auflage verantwortlich und hat zusammen mit Hans-Walter Heldt das Buch gründlich überarbeitet und aktualisiert. Neueste wissenschaftliche Erkenntnisse fanden Eingang in dieses Buch, die sich auch in neuen Abbildungen sowie der stark überarbeiteten Literatur widerspiegeln. Besonderen Wert legen die Autoren darauf, die offenen, zukunftsweisenden Fragen, die den derzeitigen Stand unseres Wissens markieren, aufzuzeigen. Aktualität sowie die klare und verständliche Didaktik komplexer Sachverhalte darzustellen -- das sind die Kennzeichen dieses Lehrbuches. Mit sorgfältig erstellten zweifarbigen Abbildungen erfüllt es einen hohen didaktischen Anspruch und reiht sich unter die besten Biochemie-Lehrbücher.
Posted in Science

Thin Layer Chromatography in Phytochemistry

Author: Monika Waksmundzka-Hajnos,Joseph Sherma,Teresa Kowalska

Publisher: CRC Press

ISBN: 9781420046786

Category: Science

Page: 896

View: 8365

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.
Posted in Science

Recent Trends in Biotechnology and Therapeutic Applications of Medicinal Plants

Author: Mohd. Shahid,Anwar Shahzad,Abida Malik,Aastha Sahai

Publisher: Springer Science & Business Media

ISBN: 9400766033

Category: Science

Page: 357

View: 4687

The book provides an overview of current trends in biotechnology and medicinal plant sciences. The work includes detailed chapters on various advance biotechnological tools involved in production of phytoactive compounds of medicinal significance. Some recent and novel research studies on therapeutic applications of different medicinal plants from various geographical regions of the world have also been included. These studies report the antimicrobial activity of various natural plant products against various pathogenic microbial strains. Informative chapters on recent emerging applications of plant products such as source for nutraceuticals and vaccines have been integrated to cover latest advances in the field. This book also explores the conservation aspect of medicinal plants. Thus, chapters having comprehensively complied in vitro conservation protocols for various commercially important rare, threatened and endangered medicinal plants were provided in the present book.
Posted in Science

High Performance Liquid Chromatography in Phytochemical Analysis

Author: Monika Waksmundzka-Hajnos,Joseph Sherma

Publisher: CRC Press

ISBN: 9781420092615

Category: Science

Page: 996

View: 5139

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a complete description of the techniques, materials, and instrumentation of column HPLC and its application to essentially all primary and secondary plant metabolites. Hailing from around the world and with vast expertise in HPLC phytochemical analysis, the contributors present a global, authoritative view of the field. The book looks at the role of HPLC in the analysis of herbal drugs, quality control of plant products in dietary supplements, and chemosystematics. It also covers the phytochemistry, pharmacology, and biological role of plant metabolites as well as various modes and techniques of HPLC analysis. The book then focuses on HPLC separation, identification, and quantification of particular classes of compounds in a variety of sample types, including plants, plant extracts, and plant-derived products. Along with its companion volume Thin Layer Chromatography in Phytochemistry, this comprehensive book presents the most important analytical approaches used in phytochemical analysis. It will help in solving problems connected with practical separations and the analyses of plant extract fractions of active metabolites.
Posted in Science