Mass Spectrometry for the Novice

Author: John Greaves,John Roboz

Publisher: CRC Press

ISBN: 1420094181

Category: Science

Page: 309

View: 3949

With usage of mass spectrometry continually expanding, an increasing number of scientists, technicians, students, and physicians are coming into contact with this valuable technique. Mass spectrometry has many uses, both qualitative and quantitative, from analyzing simple gases to environmental contaminants, pharmaceuticals, and complex biopolymers. The extraordinary versatility can make mass spectrometers daunting to novices. Consequently, new users would benefit greatly from an understanding of the basic concepts as well as the processes that occur in these instruments. Mass Spectrometry for the Novice provides exactly that, with detailed, straightforward descriptions and clear illustrations of principles of operations and techniques. The book begins with an overview that includes essential definitions and then provides information on the components of and the strategies used in the most common instruments. The authors discuss the methodologies available, classes of compounds analyzed, and the types of data that can be generated. A group of representative applications from published articles is summarized, demonstrating the diversity of mass spectrometry. The authors also condense the essentials of the topic into one invaluable chapter that provides a set of concise take-home messages on all aspects of mass spectrometry. The final section provides a collection of resources including books, reviews, and useful websites. Using simple language, new color figures, clever cartoons, and assuming no prior knowledge, this book provides a readily understandable entrée to mass spectrometry. A CD-ROM with selected figures and cartoons is included.
Posted in Science

Massenspektrometrie

Ein Lehrbuch

Author: Jürgen H Gross

Publisher: Springer-Verlag

ISBN: 3827429811

Category: Science

Page: 802

View: 7156

Mit Massenspektrometrie – ein Lehrbuch liegt ein Werk vor, das mit seiner umfassenden, präzisen Darstellung sowie seinen vielen gelungenen Illustrationen und Fotos eine Lücke auf dem deutschsprachigen Markt schließt. Dieses im englischsprachigen Raum bereits gut etablierte Buch führt auf grundlegende Weise an die Massenspektrometrie heran, indem es die Prinzipien, Methoden und Anwendungen logisch aufeinander aufbauend erklärt. Schritt für Schritt lernt der Leser, was diese analytische Methode leisten kann, auf welch vielfältige Art Massenspektrometer isolierte Ionen in der Gasphase erzeugen, selektieren und manipulieren können und wie man aus den resultierenden Massenspektren analytische Information gewinnt. Moderne sanfte Ionisationsmethoden wie ESI, APCI oder MALDI, klassische Verfahren wie EI, CI, FAB oder FD, Oberflächentechniken wie DESI oder DART und elementmassenspektrometrische Verfahren werden didaktisch durchdacht behandelt. Studienanfänger werden von dem Werk ebenso profitieren wie Fortgeschrittene und Praktiker. Ergänzend zum Buch betreibt der Autor eine frei zugängliche (englischsprachige) Internetseite mit zahlreichen Übungsaufgaben, Lösungen und Bonus-Material unter http://www.ms-textbook.com
Posted in Science

Mass Spectrometry in the Biological Sciences

Author: A. L. Burlingame,Steven A. Carr

Publisher: Springer Science & Business Media

ISBN: 1461202299

Category: Science

Page: 570

View: 857

Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin. They demonstrate by example how these methodologies can solve a wide array of real-world problems in protein biochemistry, immunology, and glycobiology, as well as for human bacterial pathogens, lipids, and nucleic acids. The book offers a unique opportunity to learn these techniques that are revolutionizing the field. Its authoritative assessment in the context of how to solve important and challenging problems in bioscience and medicine ensures a competitive advantage for today's researchers.
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Blink!

Die Macht des Moments

Author: Malcolm Gladwell

Publisher: Campus Verlag

ISBN: 3593405008

Category: Science

Page: 264

View: 4993

Jeder kennt sie: Momente, in denen wir denken, ohne zu denken.Wir nennen sie Intuition oder Bauchgefühl. Aber sie sind viel mehr: Unser Gehirn arbeitet dann auf Hochtouren, um uns die richtigen Entscheidungen zu liefern. Malcolm Gladwell erklärt die Macht dieses Augenblicks und zeigt, wie wir unsere verborgene Intelligenz trainieren und besser nutzen können.
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Mass Spectrometry

A Textbook

Author: Jürgen H Gross

Publisher: Springer Science & Business Media

ISBN: 3642107095

Category: Science

Page: 753

View: 2224

This book offers a balanced mixture of practice-oriented information and theoretical background as well as numerous references, clear illustrations, and useful data tables. Problems and solutions are accessible via a special website. This new edition has been completely revised and extended; it now includes three new chapters on tandem mass spectrometry, interfaces for sampling at atmospheric pressure, and inorganic mass spectrometry.
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Gas Chromatography and Mass Spectrometry: A Practical Guide

Author: O. David Sparkman,Zelda Penton,Fulton G. Kitson

Publisher: Academic Press

ISBN: 9780080920153

Category: Science

Page: 632

View: 982

The second edition of Gas Chromatography and Mass Spectrometry: A Practical Guide follows the highly successful first edition by F.G. Kitson, B.S. Larsen, and C.N. McEwen (1996), which was designed as an indispensible resource for GC/MS practitioners regardless of whether they are a novice or well experienced. The Fundamentals section has been extensively reworked from the original edition to give more depth of an understanding of the techniques and science involved with GC/MS. Even with this expansion, the original brevity and simple didactic style has been retained. Information on chromatographic peak deconvolution has been added along with a more in-depth understanding of the use of mass spectral databases in the identification of unknowns. Since the last edition, a number of advances in GC inlet systems and sample introduction techniques have occurred, and they are included in the new edition. Other updates include a discussion on fast GC and options for combining GC detectors with mass spectrometry. The section regarding GC Conditions, Derivatization, and Mass Spectral Interpretation of Specific Compound Types has the same number of compound types as the original edition, but the information in each section has been expanded to not only explain some of the spectra but to also explain why certain fragmentations take place. The number of Appendices has been increased from 12 to 17. The Appendix on Atomic Masses and Isotope Abundances has been expanded to provide tools to aid in determination of elemental composition from isotope peak intensity ratios. An appendix with examples on "Steps to follow in the determination of elemental compositions based on isotope peak intensities" has been added. Appendices on whether to use GC/MS or LC/MS, third-party software for use in data analysis, list of information required in reporting GC/MS data, X+1 and X+2 peak relative intensities based on the number of atoms of carbon in an ion, and list of available EI mass spectral databases have been added. Others such as the ones on derivatization, isotope peak patterns for ions with Cl and/or Br, terms used in GC and in mass spectrometry, and tips on setting up, maintaining and troubleshooting a GC/MS system have all been expanded and updated. Covers the practical instruction necessary for successful operation of GC/MS equipment Reviews the latest advances in instrumentation, ionization methods, and quantitation Includes troubleshooting techniques and a variety of additional information useful for the GC/MS practitioner A true benchtop reference A guide to a basic understanding of the components of a Gas Chromatograph-Mass Spectrometer (GC-MS) Quick References to data interpretation Ready source for information on new analyses
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Inorganic Mass Spectrometry

Principles and Applications

Author: Sabine Becker

Publisher: John Wiley & Sons

ISBN: 9780470517208

Category: Science

Page: 514

View: 1621

Providing an exhaustive review of this topic, Inorganic Mass Spectrometry: Principles and Applications provides details on all aspects of inorganic mass spectrometry, from a historical overview of the topic to the principles and functions of mass separation and ion detection systems. Offering a comprehensive treatment of inorganic mass spectrometry, topics covered include: Recent developments in instrumentation Developing analytical techniques for measurements of trace and ultratrace impurities in different materials This broad textbook in inorganic mass spectrometry, presents the most important mass spectrometric techniques used in all fields of analytical chemistry. By covering recent developments and advances in all fields of inorganic mass spectrometry, this text provides researchers and students with information to answer any questions on this topic as well as providing the basic fundamentals for understanding this potentially complex, but increasingly relevant subject.
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Mass Spectrometry in Cancer Research

Author: John Roboz

Publisher: CRC Press

ISBN: 1420042696

Category: Medical

Page: 576

View: 5577

Cancer research is becoming multidisciplinary. The complex structural and therapeutic problems require synergistic approaches employing an assortment of biochemical manipulations, chromatographic or electrophoretic separations, sequencing strategies, and ... more and more mass spectrometry. Mass Spectrometry in Cancer Research provides a broad examination of current strategies and techniques and their application to the study of: (i) occupational and environmental carcinogens; (ii) antineoplastic and chemopreventive agents; (iii) pertinent proteins, lipids, nucleic acids and glycoconjugates. Also included are a chapter on instrumentation and methodologies for biologists and physicians and a brief review of the relevant concepts of cancer biology and medicine for mass spectrometrists. This book is intended for: mass spectrometrists in research or those providing core services; researchers in biological, medical, pharmaceutical or environmental sciences; physicians in academic medicine; and academic/industrial research managers.
Posted in Medical

Introducing Proteomics

From Concepts to Sample Separation, Mass Spectrometry and Data Analysis

Author: Josip Lovric

Publisher: John Wiley & Sons

ISBN: 1119957192

Category: Science

Page: 296

View: 8441

Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the following: Separation Technologies Analysis of Peptides/Proteins by Mass Spectrometry Strategies in Proteomics This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues." —Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website www.wiley.com/go/lovric
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Introduction to GC-MS Coupling

Author: Stéphane Bouchonnet

Publisher: CRC Press

ISBN: 1466572523

Category: Science

Page: 228

View: 7651

Although GC-MS (gas chromatography–mass spectrometry) finds applications in fields as diverse as the food processing industry, medicine, pharmacology, and environmental analysis, the few works that are dedicated to this use of mass spectrometry are generally highly complex and theoretical. Emphasizing the practical aspects of GC-MS, without neglecting the fundamental theory, Introduction to GC-MS Coupling addresses both novice and experienced users of this technique. It presents GC-MS in a clear, instructive way and proposes solutions for the difficulties classically encountered by users. The book begins with the core principles of gas chromatography and its specific uses with MS detectors. It discusses generalities of mass spectrometry, including the various types of MS detectors and insight into the vacuum necessary for efficient operation. Chapters cover the types of analyzers used in GC-MS and their functioning principles, with a focus on the commonly used quadrupolar analyzers, as well as the implementation, advantages, and limits of various modes of acquisition in GC-MS. The text also compares performance and limitations of quadrupolar analyzers. The author includes a full chapter on quantification using GC-MS, a topic that can be puzzling for many chemists. Encouraging a critical approach to databases, he compares laboratory-made and commercial mass spectra databases, and describes a database research algorithm. The final chapter examines mass spectra interpretation, covering chemistry concepts such as inductive and mesomeric effects required to understand dissociation pathways, and presents a global strategy for mass spectra interpretation.
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An Introduction to Spectroscopic Methods for the Identification of Organic Compounds

Nuclear Magnetic Resonance and Infrared Spectroscopy

Author: F. Scheinmann

Publisher: Elsevier

ISBN: 1483280772

Category: Science

Page: 212

View: 4705

An Introduction to Spectroscopic Methods for the Identification of Organic Compounds, Volume 1: Nuclear Magnetic Resonance and Infrared Spectroscopy discusses how spectral data can be translated into the structural formula of organic compounds and provides reference data and revised correlation tables for the initiated. The text describes high resolution nuclear magnetic resonance spectroscopy; the applications of nuclear magnetic resonance spectroscopy in organic chemistry; and correlation tables for nuclear magnetic resonance spectra. Nuclear magnetic resonance spectroscopy seminar problems and answers; the theoretical basis of infrared spectroscopy; and the applications of infrared spectroscopy to organic chemistry are also encompassed. The book further tackles infrared spectroscopic problems and answers, as well as correlation tables for infrared spectra.
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Practical Mass Spectrometry

A Contemporary Introduction

Author: Brian S. Middleditch

Publisher: Springer Science & Business Media

ISBN: 1461329825

Category: Science

Page: 388

View: 896

It has been estimated that more than 8090 of the world's scientists who have ever lived are still alive today. It would not be unreasonable to suggest that more than 95% of those who have ever used a mass spectrometer are not only alive but are still actively employed. Most have never had any formal training in the subject since, with a few notable exceptions, universities have only recently begun to offer courses in mass spectrometry. We have written this book for the student of modern mass spectro metry: it is for the novice who wished to know what the instruments can do and how the techniques can be applied. There are other books on the market which delve into the history of mass spectrometry and go deeply into the mathematical theory and instrumentation. There are yet more books which guide one through the art of interpreting spectra. We have deliberately avoided these topics so that the reader is confronted only with the basic principles and is allowed a taste of the applications. One of the best methods of deVeloping a useful textbook is to teach a course based upon its content. This is what we did. We met in Houston in 1976 to teach a course on "Perspectives in Mass Spectrometry" and to coordinate our writing. The authors of five of the chapters met again in St.
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Laborpraxis Band 2: Messmethoden

Author: aprentas

Publisher: Springer-Verlag

ISBN: 3034809689

Category: Science

Page: 119

View: 4294

Das vierbändige Standardwerk für die grundlegende praktische Arbeit im chemisch-pharmazeutischen Labor mit Schwerpunkt Synthesemethoden, Chromatographie und Spektroskopie liegt jetzt in der 6. völlig neu überarbeiteten Auflage vor. Es dient Berufseinsteigern als breit angelegtes Lehrmittel und erfahrenen Fachkräften als Nachschlagewerk mit übersichtlich dargestellten theoretischen Grundlagen und konkreten, erprobten Anwendungsideen. Die theoretischen Grundlagen für jedes Kapitel sind gut lesbar abgefasst und unterstützen das Verständnis für praktische Arbeiten und Gerätefunktionen. Zu jedem Kapitel gibt es Hinweise auf vertiefende und weiterführende Literatur. Arbeitssicherheit und –hygiene sowie die zwölf Prinzipien der nachhaltigen Chemie finden neben den entsprechenden Kapiteln Beachtung. Die im Buch erwähnten praktischen Grundlagen gründen auf Gegebenheiten in der chemisch-pharmazeutischen Industrie in der Schweiz. Sie finden im gesamten deutschsprachigen Raum Anwendung, auch in verwandten Arbeitsgebieten wie biochemischen, klinischen, werkstoffkundlichen oder universitären Laboratorien. Die Laborpraxis eignet sich für den Einsatz in der Grund- und in der Weiterbildung von Fachpersonal. Der Inhalt entspricht den aktuellen Anforderungen der Bildungsverordnung und des Bildungsplanes zum Beruf Laborantin / Laborant mit eidgenössischem Fähigkeitszeugnis (EFZ), welche vom Staatssekretariat für Bildung, Forschung und Innovation (SBFI) in Bern verordnet wurden. Damit steht den Lernenden eine gute Grundlage für die Vorbereitung auf das Qualifikationsverfahren (QV) zur Verfügung; Expertinnen und Experten stützen ihre persönlichen Vorbereitungsarbeiten und ihre fachlichen Beurteilungen auf dieses Buch. Band 1 und Band 2 behandeln ausführlich die Grundlagen der Laborarbeit und ausgewählte Messtechniken. Band 3 ist auf präparative und analytische Trennungsmethoden, Band 4 auf nasschemische und spektroskopische Analysemethoden fokussiert. Sowohl konventionelle Methoden als auch modernste Techniken finden Erwähnung. Der Fokus auf beständig gültigen Prinzipien erlaubt auch neue, nicht erwähnte Techniktrends zu verstehen sowie die tägliche Arbeit im chemisch-pharmazeutischen Labor zu reflektieren.
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Practical Guide to ICP-MS

A Tutorial for Beginners, Second Edition

Author: Robert Thomas

Publisher: CRC Press

ISBN: 9781420067873

Category: Science

Page: 376

View: 6778

Whatever your ICP-MS experience, you probably know that there are many textbooks compiled and edited by academics that approach ICP-MS from a purely theoretical and fundamental perspective, but there aren’t any books that provide a practical perspective of the technique that are written specifically for the novice user. You’ll be glad to know that the landscape is changing. With the publication of Practical Guide to ICP-MS: A Tutorial for Beginners, Second Edition, the user community has an new reference book that covers everything a beginner needs to know. Aimed specifically at analytical chemists who are not familiar with the technique and novice users who have just started using it, this updated, revised, and rewritten manuscript includes: Updated sections on ICP-MS basic principles to reflect advancements in the technology over the past four years New chapter on Trace Element Speciation using HPLC-ICP-MS New comprehensive easy-to-understand Glossary/dictionary of ICP-MS terms Extensive revisions to the chapter on "How to Select an ICP-MS" Applications section incorporates solutions to more and diverse application problems Rewritten chapter on collision/reaction cells and interfaces takes a detailed look at the different commercial approaches Updated chapter on alternative sample introduction techniques, including all the new commercially-available systems Revised chapter on comparing ICP-MS with other Atomic Spectroscopy techniques to reflect the most recent developments Written by an insider with more than 20 years experience in product development, customer support, and technical marketing for an ICP-MS instrument vendor, the book highlights this powerful ultra trace-element technique as a practical solution to real-world problems. The in-depth presentation of analytical advantages and practical capabilities of the technique from someone who has been involved in all aspects of ICP-MS instrumental analysis, gives the author a unique insight into the technique and therefore makes the book an important part of any laboratory toolkit.
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Microchip Capillary Electrophoresis

Methods and Protocols

Author: Charles Sherman Henry

Publisher: Springer Science & Business Media

ISBN: 1597450766

Category: Science

Page: 237

View: 9656

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. "Microchip Capillary Electrophoresis: Methods and Protocols" provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.
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Mitteilungsblatt

Author: Gesellschaft Deutscher Chemiker. Fachgruppe Analytische Chemie

Publisher: N.A

ISBN: N.A

Category: Chemistry, Analytic

Page: N.A

View: 6240

Posted in Chemistry, Analytic

The Maillard Reaction

Author: S. E. Fayle,Juliet A. Gerrard,Royal Society of Chemistry (Great Britain)

Publisher: Royal Society of Chemistry

ISBN: 9780854045815

Category: Science

Page: 120

View: 6851

This unique book provides a 'one-stop' text from which methods of analysis of Maillard products may be obtained.
Posted in Science

Journal

Author: American Chemical Society

Publisher: N.A

ISBN: N.A

Category: Chemistry

Page: N.A

View: 2413

Posted in Chemistry