Immunochemical Methods in Cell and Molecular Biology

Author: N.A

Publisher: Academic Press

ISBN: 9780124808553

Category: Science

Page: 227

View: 9125

This book presents up-to-date immunochemical techniques for isolating and analyzing a wide variety of biological products. It collates existing information in one source, presenting important basic and applied advances and provides a stimulating forum for the discussion of new ideas and observations on genetically manipulated microbial and viral agents.
Posted in Science

Principles and Techniques of Biochemistry and Molecular Biology

Author: Keith Wilson,John Walker

Publisher: Cambridge University Press

ISBN: 1107393493

Category: Science

Page: N.A

View: 9519

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.
Posted in Science

Methods in Plant Biochemistry and Molecular Biology

Author: William V. Dashek

Publisher: CRC Press

ISBN: 1351091387

Category: Science

Page: 480

View: 4473

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.
Posted in Science

Handbook of Immunochemistry

Author: Miroslav Ferenčík

Publisher: Springer Science & Business Media

ISBN: 9401115524

Category: Science

Page: 519

View: 719

The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.
Posted in Science

Molecular Biology in Marine Science

Scientific Questions, Technological Approaches, and Practical Implications

Author: N.A

Publisher: National Academies

ISBN: N.A

Category: Marine sciences

Page: 76

View: 1642

Posted in Marine sciences

Antibodies in Cell Biology

Author: N.A

Publisher: Academic Press

ISBN: 9780080859354

Category: Medical

Page: 452

View: 5908

Antibodies in Cell Biology focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and troubleshooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas. Practical applications and future emphases of antibodies, including: Light microscopic immunolocalization of antigens Gold particles in immunoelectron microscopy Special methods of fixation and permeabilization Microinjection of antibodies into living cells Antibodies to identify cDNA clones Antisense antibody strategies
Posted in Medical

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics - E-Book

Author: Carl A. Burtis,Edward R. Ashwood,David E. Bruns

Publisher: Elsevier Health Sciences

ISBN: 1455759422

Category: Medical

Page: 2256

View: 4777

As the definitive reference for clinical chemistry, Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th Edition offers the most current and authoritative guidance on selecting, performing, and evaluating results of new and established laboratory tests. Up-to-date encyclopedic coverage details everything you need to know, including: analytical criteria for the medical usefulness of laboratory procedures; new approaches for establishing reference ranges; variables that affect tests and results; the impact of modern analytical tools on lab management and costs; and applications of statistical methods. In addition to updated content throughout, this two-color edition also features a new chapter on hemostasis and the latest advances in molecular diagnostics. Section on Molecular Diagnostics and Genetics contains nine expanded chapters that focus on emerging issues and techniques, written by experts in field, including Y.M. Dennis Lo, Rossa W.K. Chiu, Carl Wittwer, Noriko Kusukawa, Cindy Vnencak-Jones, Thomas Williams, Victor Weedn, Malek Kamoun, Howard Baum, Angela Caliendo, Aaron Bossler, Gwendolyn McMillin, and Kojo S.J. Elenitoba-Johnson. Highly-respected author team includes three editors who are well known in the clinical chemistry world. Reference values in the appendix give you one location for comparing and evaluating test results. NEW! Two-color design throughout highlights important features, illustrations, and content for a quick reference. NEW! Chapter on hemostasis provides you with all the information you need to accurately conduct this type of clinical testing. NEW! Six associate editors, Ann Gronowski, W. Greg Miller, Michael Oellerich, Francois Rousseau, Mitchell Scott, and Karl Voelkerding, lend even more expertise and insight to the reference. NEW! Reorganized chapters ensure that only the most current information is included.
Posted in Medical

Epidermal Cells

Methods and Protocols

Author: Kursad Turksen

Publisher: Springer Science & Business Media

ISBN: 1592598307

Category: Science

Page: 480

View: 536

Helps the reader to learn about the derivation, characterization, and utility of epidermal stem cells; follow step-by-step instructions that ensure successful results; understand the utility of epidermal cells in regenerative medicine applications; and apply reproducible methods to study epidermal precursors and mature epidermal cells.
Posted in Science

Molecular and Cellular Methods in Developmental Toxicology

Author: George P. Daston

Publisher: CRC Press

ISBN: 9781439810668

Category: Science

Page: 304

View: 8577

This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.
Posted in Science

Methods in Cell Biology

Author: N.A

Publisher: Academic Press

ISBN: 9780080858999

Category: Science

Page: 425

View: 8709

Methods in Cell Biology
Posted in Science

Immunochemical Protocols

Author: John Pound

Publisher: Springer Science & Business Media

ISBN: 1592592570

Category: Medical

Page: 508

View: 7711

This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Posted in Medical

Cellular and Molecular Immunology E-Book

Author: Abul K. Abbas,Andrew H. H. Lichtman,Shiv Pillai

Publisher: Elsevier Health Sciences

ISBN: 0323286453

Category: Medical

Page: 544

View: 6998

Popular for its highly visual, straightforward approach, Cellular and Molecular Immunology delivers an accessible yet thorough understanding of this active and fast-changing field. Drs. Abul K. Abbas, Andrew H. Lichtman, and Shiv Pillai present key updates in this new edition to cover the latest developments in antigen receptors and signal transduction in immune cells, mucosal and skin immunity, cytokines, leukocyte-endothelial interaction, and more. With additional online features, this is an ideal resource for medical, graduate and undergraduate students of immunology who need a clear, introductory text for immunology courses. Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Develop a thorough, clinically relevant understanding of immunology through a clear overview of immunology with a distinct focus on the management of human disease. Visualize immunologic processes more effectively. Meticulously developed and updated illustrations, 3-dimensional art, and all-new animations provide a detailed, visual description of the key immunologic and molecular processes. Grasp the details of experimental observations that form the basis for the science of immunology at the molecular, cellular, and whole-organism levels and draw the appropriate conclusions. Find information more quickly and easily through an organized chapter structure and a more logical flow of material. Glean all essential, up-to-date, need-to-know information about immunology and molecular biology through extensive updates that cover cytokines, innate immunity, leukocyte-endothelial interactions, signaling, costimulation, and more. Benefit from numerous new figures and tables that facilitate easier retention of the material; quick summaries of each chapter; and nearly 400 illustrations that clarify key concepts.
Posted in Medical

Biochemical Roles of Eukaryotic Cell Surface Macromolecules

2011 ISCSM Proceedings

Author: Perumana R. Sudhakaran,Avadhesha Surolia

Publisher: Springer Science & Business Media

ISBN: 1461433819

Category: Science

Page: 340

View: 4212

Cell surface molecules are critically important in regulating cell structure and function. Recent advances on the functional role of cell surface molecules, particularly glycoconjugates are presented in this book. Comprising of 22 chapters from the 2011 International Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules, it covers topics on the analysis of glycome, biophysical approaches to study cell surface molecules, glycoconjugate metabolism and its dysregulation, and molecular mechanisms involved in cell-cell and cell–matrix interaction.
Posted in Science

Principles and Techniques of Practical Biochemistry

Author: Keith Wilson,John Walker

Publisher: Cambridge University Press

ISBN: 9780521658737

Category: Medical

Page: 784

View: 858

New edition of biochemistry textbook which introduces principles and techniques used in undergraduate practical classes.
Posted in Medical

Thiol Redox Transitions in Cell Signaling, Part A

Chemistry and Biochemistry of Low Molecular Weight and Protein Thiols

Author: N.A

Publisher: Academic Press

ISBN: 9780123813466

Category: Science

Page: 416

View: 1701

Thiol Redox Transitions in Cell Signaling, Part A, along with its companion (volume 475), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. This first installment of Cadenas and Packer's two-volume treatment specifically deals with glutathionylation and dethiolation, and peroxide removal by peroxiredoxins/thioredoxins and glutathione peroxidases. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Posted in Science

Biochemical Roles of Eukaryotic Cell Surface Macromolecules

Author: Abhijit Chakrabarti,Avadhesha Surolia

Publisher: Springer

ISBN: 3319112805

Category: Science

Page: 411

View: 6832

Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: · neurochemical and biochemical analysis of cell surface glycoconjugates · membrane skeletal organization · GPCRs and other receptors · biophysical approaches to study membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms involved in cell-cell and cell-matrix interaction · glycans in infectious and neurological diseases · cancer and glycosyltransferases as drug targets.
Posted in Science

Methods in Cartilage Research

Author: Alice Maroudas,Klaus E. Kuettner

Publisher: N.A

ISBN: N.A

Category: Articular cartilage

Page: 370

View: 2191

This book details and critically evaluates a wide variety of techniques used in cartilage research, ranging from biophysics and biomechanics to biochemistry and molecular biology, with special reference to degenerative diseases. With contributions from over a hundred leading experts and nearly one thousand key references this work will be indispensable to cell biologists, physiologists, pathologists, rheumatologists and others working in cartilage biology. The methods described include: * sampling, characterization and handling of tissue specimens * extraction, separation and analysis of matrix constituents * modern morphological techniques * isolated chondrocyte culture * explant culture of tissue segments * use of radioisotopes in metabolic studies of matrix macromolecules * immunochemical methods in cartilage research * contemporary molecular biology techniques * quantitative aspects of cartilage composition * measurement of solute transport * measurement of physicochemical, mechanical and electromechanical properties * cartilage repair and modeling * degradation products as markers of cartilage metabolism and disease
Posted in Articular cartilage

Thiol Redox Transitions in Cell Signaling

Author: N.A

Publisher: Academic Press

ISBN: 9780123810045

Category: Science

Page: 392

View: 3950

This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Posted in Science