Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications

Author: R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 1118873378

Category: Science

Page: 728

View: 573

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Posted in Science

Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications

Author: R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 1118873645

Category: Science

Page: 728

View: 8729

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Posted in Science

Culture of Animal Cells

A Manual of Basic Technique and Specialized Applications

Author: R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 9780470649350

Category: Science

Page: 768

View: 3634

This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture
Posted in Science

General Techniques of Cell Culture

Author: Maureen A. Harrison,Ian F. Rae

Publisher: Cambridge University Press

ISBN: 9780521574969

Category: Science

Page: 162

View: 3433

Concise introduction to a major technique of cell biology laboratories for those new to the field.
Posted in Science

Culture of Animal Cells, A Manual of Basic Technique and Specialized Applications

Biology, Cell biology

Author: CTI Reviews

Publisher: Cram101 Textbook Reviews

ISBN: 1478419024

Category: Education

Page: 50

View: 8993

Facts101 is your complete guide to Culture of Animal Cells, A Manual of Basic Technique and Specialized Applications. In this book, you will learn topics such as as those in your book plus much more. With key features such as key terms, people and places, Facts101 gives you all the information you need to prepare for your next exam. Our practice tests are specific to the textbook and we have designed tools to make the most of your limited study time.
Posted in Education

Animal Cell Culture

Essential Methods

Author: John M. Davis

Publisher: John Wiley & Sons

ISBN: 9780470975633

Category: Science

Page: 374

View: 2006

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.
Posted in Science

Animal Cell Culture and Technology

Author: Michael Butler

Publisher: Garland Science

ISBN: 1135325375

Category: Science

Page: 256

View: 6082

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.
Posted in Science

Human Stem Cell Manual

A Laboratory Guide

Author: Suzanne Peterson,Jeanne F. Loring

Publisher: Academic Press

ISBN: 0123854741

Category: Science

Page: 656

View: 9383

This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications. Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America. Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs.
Posted in Science

Culture of Human Tumor Cells

Author: Roswitha Pfragner,R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 0471270628

Category: Science

Page: 452

View: 8493

This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.
Posted in Science

Animal Cell Culture Techniques

Author: Martin Clynes

Publisher: Springer

ISBN: 9783540630081

Category: Science

Page: 618

View: 8831

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Posted in Science

Protein Purification

Author: Philip L.R. Bonner

Publisher: Garland Science

ISBN: 0203967267

Category: Science

Page: 230

View: 5159

Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Posted in Science

Basic Cell Culture

A Practical Approach

Author: John M. David

Publisher: Oxford University Press

ISBN: 9780199634330

Category: Science

Page: 301

View: 9991

The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.
Posted in Science

Calculations for Molecular Biology and Biotechnology

Author: Frank H. Stephenson

Publisher: Academic Press

ISBN: 0128025980

Category: Computers

Page: 496

View: 1518

Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts
Posted in Computers

Culture of Cells for Tissue Engineering

Author: Gordana Vunjak-Novakovic,R. Ian Freshney

Publisher: John Wiley & Sons

ISBN: 0471741809

Category: Science

Page: 352

View: 3372

Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure. Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book A section of elegant color plates to back up the figures in the chapters Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.
Posted in Science

A Dissection and Tissue Culture Manual of the Nervous System

Author: Abraham Shahar

Publisher: Wiley-Liss

ISBN: N.A

Category: Medical

Page: 371

View: 2038

At last . a collection of practical protocols for explanting and manipulating neuronal and glial cells. A Dissection and Tissue Culture Manual of the Nervous System Abraham Shahar, Jean de Vellis, Antonia Vernadakis, and Bernard Haber, Editors Among research laboratories involved with neuronal and glial cell cultures and their applications, there is a growing demand for a hand-book describing dissection procedures, culture preparation techniques, and the in vitro manipulation of neural cells and tissues for specific analytical purposes. A Dissection and Tissue Culture Manual of the Nervous System offers a diverse collection of methods that have been developed by and are used routinely within specialized neurobiological laboratories. Written in an easy-to-follow style, the procedures described in this unique guide are designed by experts to be applied by those with limited experience in the field. Organized into ten comprehensive sections, ninety concise contributions from leading laboratories worldwide put forth practical, stepwise protocols for neural cell manipulation and experimentation. Methods encompass: * an illustrated outline of techniques for the dissection of brain areas in the fetus and the neonate * the dissection of selected specialized structures, such as the ciliary ganglion * organotypic. explant culture of nervous tissue * dissociated culture of astrocytes, oligodendrocyte, neurons, and Schwann cells * reaggregation culture of dissociated cells. Sections devoted to various tissue processing methods and experimental applications of cultured material present histochemical, autoradio-graphic, and immunocytochemical staining and visualization techniques. In situ hybridization methods, as well as preparative procedures for electron microscopy and biochemical and physiological assays, are discussed with an emphasis on methods tailored for the neurobiologist. Alternative techniques for the cultivation of the same organ or cell type from diverse animal species are juxtaposed with a varied selection of methodology and instrumentation, and complemented by key literature citations for further reading, to enable the investigator to chose the appropriate approach for a specific neurobiological application. Presented in a comb-bound format for convenient use on the laboratory bench, A Dissection and Tissue Culture Manual of the Nearvous System will be an essential research companion to graduate students, post-doctoral fellows and other laborabory investigators in cell and developmental neurobiology, neuroanatomy, neurophysiology, neuropharmacology, and biochemistry.
Posted in Medical

Guide to Research Techniques in Neuroscience

Author: Matt Carter,Jennifer C. Shieh

Publisher: Academic Press

ISBN: 0128005971

Category: Science

Page: 418

View: 988

Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments. This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines. Written to provide a "hands-on" approach for graduate students, postdocs, or anyone new to the neurosciences Techniques within one field are compared, allowing readers to select the best techniques for their own work Includes key articles, books, and protocols for additional detailed study Data analysis boxes in each chapter help with data interpretation and offer guidelines on how best to represent results Walk-through boxes guide readers step-by-step through experiments
Posted in Science

Extreme Tissue Engineering

Concepts and Strategies for Tissue Fabrication

Author: Robert A. Brown

Publisher: John Wiley & Sons

ISBN: 1119942667

Category: Technology & Engineering

Page: 272

View: 2421

Highly Commended at the BMA Book Awards 2013 Extreme Tissue Engineering is an engaging introduction to Tissue Engineering and Regenerative Medicine (TERM), allowing the reader to understand, discern and place into context the mass of scientific, multi-disciplinary data currently flooding the field. It is designed to provide interdisciplinary, ground-up explanations in a digestible, entertaining way, creating a text which is relevant to all students of TERM regardless of their route into the field. Organised into three main sections: chapters 1 to 3 introduce and explain the general problems; chapters 4 to 6 identify and refine how the main factors interact to create the problems and opportunities we know all too well; chapters 7 to 9 argue us through the ways we can use leading-edge (extreme) concepts to build our advanced solutions. Students and researchers in areas such as stem cell and developmental biology, tissue repair, implantology and surgical sciences, biomaterials sciences and nanobiomedicine, bioengineering, bio-processing and monitoring technologies - from undergraduate and masters to doctoral and post-doctoral research levels - will find Extreme Tissue Engineering a stimulating and inspiring text. Written in a fluid, entertaining style, Extreme Tissue Engineering is introductory yet challenging, richly illustrated and truly interdisciplinary.
Posted in Technology & Engineering

Membrane Structural Biology

With Biochemical and Biophysical Foundations

Author: Mary Luckey

Publisher: Cambridge University Press

ISBN: 1107729335

Category: Science

Page: 400

View: 3710

This textbook provides a strong foundation and a clear overview for students of membrane biology and an invaluable synthesis of cutting-edge research for working scientists. The text retains its clear and engaging style, providing a solid background in membrane biochemistry, while also incorporating the approaches of biophysics, genetics and cell biology to investigations of membrane structure, function and biogenesis to provide a unique overview of this fast-moving field. A wealth of new high resolution structures of membrane proteins are presented, including the Na/K pump and a receptor-G protein complex, offering exciting insights into how they function. All key tools of current membrane research are described, including detergents and model systems, bioinformatics, protein-folding methodology, crystallography and diffraction, and molecular modeling. This comprehensive and up-to-date text, emphasising the correlations between membrane research and human health, provides a solid foundation for all those working in this field.
Posted in Science

The Cell

Biochemistry, Physiology, Morphology

Author: Jean Brachet,Alfred E. Mirsky

Publisher: Elsevier

ISBN: 1483216543

Category: Medical

Page: 840

View: 8532

The Cell: Biochemistry, Physiology, Morphology, Volume I focuses on the advancements of processes, techniques, and approaches used in studies on the structure and functions of cells. The selection first offers information on the optical methods in cytology, fixation and staining, and autoradiography. Discussions focus on microscopical analysis, techniques related to autoradiography, staining for electron microscopy and light microscopy, fixation, electron microscope in cytology, and X-ray microscopy of cells. The text then takes a look at the quantitative microscopical techniques for single cells and quantitative microchemical techniques of histo- and cytochemistry. The manuscript ponders on micrurgical studies on living cells, isolation of subcellular components, and "tissue culture," cellular autonomy, and cellular interrelations. Topics include standardization procedures, factors influencing the choice of isolation procedure, future applications and extensions of micrurgy, and applications of micrurgy to the study of living cells. The effects of radiations on cells, acquisition of biological specificity, and nucleocytoplasmic interactions in eggs and embryos are also discussed. The selection is a dependable reference for researchers interested in pursuing further studies on the biochemistry, physiology, and morphology of cells.
Posted in Medical

Tissue Culture

Methods and Applications

Author: Paul F. Jr. Kruse

Publisher: Elsevier

ISBN: 0323142079

Category: Science

Page: 896

View: 7444

Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Posted in Science