Crispr-Cas: A Laboratory Manual

Author: Jennifer Doudna,Prashant Mali

Publisher: N.A

ISBN: 9781621821311

Category: Medical

Page: 182

View: 8192

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign DNA, these powerful techniques allow investigators to edit DNA sequences and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system-especially for minimizing off-target effects-are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.
Posted in Medical

CRISPR-Cas

Author: University Jennifer Doudna,Prashant Mali

Publisher: N.A

ISBN: 9781621821304

Category: RNA

Page: 192

View: 9142

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.
Posted in RNA

RNA

A Laboratory Manual

Author: Donald Charles Rio

Publisher: N.A

ISBN: 9780879698904

Category: Science

Page: 586

View: 1389

Almost all molecular and cellular biology laboratories now handle RNA and this manual is an authoritative source of information and protocols for this purpose, from the basic to the advanced. Required reading for every research laboratory in the life sciences.
Posted in Science

Live Cell Imaging

A Laboratory Manual

Author: Robert D. Goldman,David L. Spector

Publisher: CSHL Press

ISBN: 9780879696832

Category: Science

Page: 631

View: 7768

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Posted in Science

The Condensed Protocols from Molecular Cloning : a Laboratory Manual

Author: Joseph Sambrook,David William Russell

Publisher: CSHL Press

ISBN: 0879697717

Category: Science

Page: 800

View: 3745

The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Posted in Science

Proteomics in Practice

A Guide to Successful Experimental Design

Author: Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker

Publisher: John Wiley & Sons

ISBN: 3527622306

Category: Science

Page: 502

View: 1068

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Posted in Science

Modern Prometheus

Author: Jim Kozubek

Publisher: Cambridge University Press

ISBN: 1108454623

Category: Law

Page: 478

View: 4119

This book tells the dramatic story of Crispr and the potential impact of this gene-editing technology.
Posted in Law

Mouse Hematology

A Laboratory Manual

Author: Michael P. McGarry,Cheryl A. Protheroe,James J. Lee

Publisher: N.A

ISBN: 9780879698850

Category: Science

Page: 99

View: 3619

Mice are used extensively in studies of blood, the immune system, and inflammation. This book is a concise review of conventional methods for preparing and examining blood and blood-forming tissues. It contains a short laboratory manual featuring detailed protocols, a DVD of video demonstrations, and a poster of blood cell types seen microscopically.
Posted in Science

Purifying Proteins for Proteomics

A Laboratory Manual

Author: Richard J. Simpson

Publisher: N.A

ISBN: 9780879696955

Category: Science

Page: 801

View: 2148

This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.
Posted in Science

Antibodies

A Laboratory Manual

Author: Edward Harlow,David Lane

Publisher: CSHL Press

ISBN: 9780879693145

Category: Science

Page: 726

View: 3540

Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.
Posted in Science

Molecular Cloning

A Laboratory Manual

Author: Joseph Sambrook,David William Russell

Publisher: CSHL Press

ISBN: 9780879695774

Category: Science

Page: 2344

View: 1041

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
Posted in Science

A Crack in Creation

The New Power to Control Evolution

Author: Jennifer Doudna,Samuel Sternberg

Publisher: Vintage

ISBN: 9781784702762

Category:

Page: 304

View: 1237

A handful of discoveries have changed the course of human history. This book is about the most recent and potentially the most powerful and dangerous of them all. It is an invention that allows us to rewrite the genetic code that shapes and controls all living beings with astonishing accuracy and ease. Thanks to it, the dreams of genetic manipulation have become a stark reality: the power to cure disease and alleviate suffering, to create new sources of food and energy, as well as to re-design any species, including humans, for our own ends. Jennifer Doudna is the co-inventor of this technology - known as CRISPR - and a scientist of worldwide renown. Writing with fellow researcher Samuel Sternberg, here she provides the definitive account of her discovery, explaining how this wondrous invention works and what it is capable of. She also asks us to consider what our new-found power means: how do we enjoy its unprecedented benefits while avoiding its equally unprecedented dangers? The future of humankind - and of all life on Earth - is at stake. This book is an essential guide to the path that now lies ahead.
Posted in

CRISPR

Methods and Protocols

Author: Magnus Lundgren,Emmanuelle Charpentier,Peter C. Fineran

Publisher: Humana Press

ISBN: 9781493926862

Category: Medical

Page: 366

View: 3353

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
Posted in Medical

Phage Display

Author: Carlos F. Barbas,Dennis R. Burton,Jamie K. Scott,Gregg J. Silverman

Publisher: CSHL Press

ISBN: 0879697407

Category: Science

Page: 736

View: 8119

Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Posted in Science

The Systematic Identification of Organic Compounds

Author: Ralph L. Shriner,Christine K. F. Hermann,Terence C. Morrill,David Y. Curtin,Reynold C. Fuson

Publisher: Wiley

ISBN: 9780471215035

Category: Science

Page: 736

View: 5462

Dedicated to qualitative organic chemistry, this book explains how to identify organic compounds through step-by-step instructions. Topics include elemental analysis, solubility, infrared, nuclear magnetic resonance and mass spectra; classification tests; and preparation of a derivative. Most directions for experiments are described in micro or mini scales. Discusses chromatography, distillations and the separation of mixtures. Questions and problems emphasize the skills required in identifying unknown samples.
Posted in Science

Single-molecule Techniques

A Laboratory Manual

Author: Paul R. Selvin,Taekjip Ha

Publisher: CSHL Press

ISBN: 087969775X

Category: Science

Page: 507

View: 9767

As molecular and cellular biologists move toward nano-techniques for performing experiments on single molecules rather than on populations of molecules, a comprehensive manual on how (and why) to carry out such experiments is needed. Single-Molecule Techniques: A Laboratory Manualfills this requirement – it is the first to take researchers who know nothing about single-molecule analyses to the point where they can successfully design and execute appropriate experiments. Geared toward research scientists in structural and molecular biology, biochemistry, and biophysics, the manual will be useful to all who are interested in observing, manipulating, and elucidating the molecular mechanisms and discrete properties of macromolecules. Techniques range from in vivo and in vitro fluorescent-based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. The book is edited by Paul R. Selvin and Taekjip Ha, two pioneers in the field of experimental biophysics who have made significant contributions to the development and application of single-molecule technologies.
Posted in Science

Antibodies

A Laboratory Manual

Author: Edward A. Greenfield

Publisher: Anchor Books

ISBN: 9781936113804

Category: Science

Page: 847

View: 6333

"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"--
Posted in Science

PCR primer

a laboratory manual

Author: Carl W. Dieffenbach,Gabriela S. Dveksler

Publisher: Cold Spring Harbor Laboratory Pr

ISBN: N.A

Category: Business & Economics

Page: 520

View: 7819

This second edition of a much-praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.
Posted in Business & Economics

Protein-protein Interactions

A Molecular Cloning Manual

Author: Erica Golemis

Publisher: CSHL Press

ISBN: 0879697237

Category: Science

Page: 938

View: 3460

Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.
Posted in Science

Methods in plant molecular biology

a laboratory course manual

Author: P. Maliga

Publisher: Cold Spring Harbor Laboratory Pr

ISBN: N.A

Category: Science

Page: 446

View: 8208

Posted in Science